We are one of the leaders of consultation in cutting edge technology related to next-generation sequencing and analysis. In this area, we are working in several fields to examine DNA sequencing, analyze data, and provide solutions to genetic mutations and personalised medicine. However, this is just a start. Our team of researchers and students are working in a wide array of areas related to designs and sequencing, including:
Specific Primer Design
Primer design is as much of an art as it is a science. Developing the right design takes real talent, and our team of scientists and researchers have developed the skill and expertise to create specific Primer designs for gene specific PCR, ARMS PCR, and multiplex PCR.
Multiplex Primer Design
Our scientists have become experts at developing multiplex Primer design that assists and experimental workflow for simultaneous detection of various genes without non-specific amplicons. The multiplicity can be used across a wide range of applications, including medical diagnosis, testing, and treatment.
ARMS PCR Design
This design uses primers in a single PCR that assists in simple and economical methods for determining and analyzing genotypes single-nucleotide polymorphisms. Our team has developed ARMS PCR designs that change reagent concentrations affecting PCR, helping to balance the inner primers band.
Real Time PCR Design
Using online tools and software programs, we are able to design primers and probes that are used in real-time PCR experiments. This allows results to be determined quickly, giving researchers the ability to customize any criterion to optimize results for more successful analysis and treatment.
SNP Analysis
Our team has been doing extensive work using multiple sequence alignment tools. This has helped us to further advance analysis related to single nucleotide polymorphism (SNP) as well as mutation analysis. Using various software packages, we are able to calculate the probability for each of the SNPs.
Mutation Validation Service
As part of our work in SNP analysis, we have been working to find and validate mutation points related to single nucleotide polymorphisms and indels. This has led us to find dozens of novel mutations in the gene. Our goal is to continue this work to find variation and validate their existence observed using next generations sequencing
Genotyping Analysis Service
We are working in detecting genetic differences that can lead to significant changes in the phenotype. This includes pathological changes that lead to underlying diseases. Through this research, we have been able to analyze the impact of genotyping and provide solutions and disease associated pathways for continuing research. We have made significant breakthroughs in SNP genotyping, focusing on four primary areas association with diseases, agricultural traits, population genomics, and microorganisms.
Haplotyping Analysis Service
Haplotyping has become an exciting area in the field of genetic sequencing and analysis. We have been on the cutting edge of physically grouping genomic variance that have been found to be inherited together. Through our research, we have discovered several haplotype groups along single chromosomes and have determined the impact of these groups have. While much research is still needed to be conducted, we are working to find real-world solutions through analyzing these group variants.
Bacterial identification - 16s rRNA Sequencing and Analysis
Through our work in sequencing 16s rRNA, we have been able to assess microbial diversity, determining the sequence, and analyzing sequencing and variance to determine their impact. This has helped us to design additional sequencing primers, conducting experiments on how this would impact communities, both imagined and real. This is an exciting field that we are participating in, both conducting research and working with others in sequencing and analysis.
Bacterial Whole Genome Sequencing
We are working on whole genome sequencing for bacteria. The purpose is not only to find the genetic codes for various bacteria, but is focused on pathogens, looking to analyze and find solutions to diseases and illnesses. Our researchers and students are dedicated to analyzing the genetic sequences of these bacteria, performing intense analysis on drug-resistant bacteria and how to overcome the obstacle of their resistance. This has led to some fascinating research related to the whole genome sequence specifically on resistant genes and biosynthetic gene clusters for novel drug discovery.
Whole Mitochondrial Genome Sequencing
Whole mitochondrial genome sequencing focuses on the genetic variation of mtDNA among populations. The sequencing can help to screen for potential disease pathogens and discover genes susceptible to illnesses.
By discovering the genetic mechanism for these diseases, their evolution, and analyzing them in comparison to population genetics, we are working to overcome common diseases and pathogens that are driven by genome sequencing and variations.
Whole mitochondrial genome sequencing can also be used for the molecular taxonomy of insects in the field of entomology in additions with classical taxonomy.
Biomarkers Design and Validation
One of the fascinating areas of our research is biomarker design and validation. These biomarkers are used to provide a putative value to be used during clinical trials. This helps in the diagnostic and enrichment process. We are focused on creating biomarkers, especially in unmet medical areas where there has been a lack of traction, feasibility, or objective endpoints. Our goal is to shine light on some of these biomarkers that the medical community has failed to focus on.
